ARG22714
anti-CD25 antibody [KEI-alpha1]
anti-CD25 antibody [KEI-alpha1] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Rabbit
Immune System antibody; Pre-B Cell Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [KEI-alpha1] recognizes CD25 This antibody recognizes Rabbit CD5, a 55kDa cell surface antigen, otherwise known as the interleukin-2 (IL-2) receptor alpha chain. In Rabbits, CD25 is expressed on activated T lymphocytes. Mouse anti Rabbit CD25 antibody, clone KEI-alpha1 is reported to inhibit the binding of IL-2 to its receptor (Kotani et al. 1993). |
|---|---|
| Tested Reactivity | Rb |
| Species Does Not React With | Hu |
| Tested Application | FACS, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | KEI-alpha1 |
| Isotype | IgG2b |
| Target Name | CD25 |
| Antigen Species | Rabbit |
| Immunogen | Cell membrane preparation of HTLV-1 transformed rabbit T cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL2RA |
|---|---|
| Gene Full Name | interleukin 2 receptor, alpha |
| Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] |
| Function | Receptor for interleukin-2. [UniProt] |
| Research Area | Immune System antibody; Pre-B Cell Marker antibody |
| Calculated MW | 31 kDa |
Images (1) Click the Picture to Zoom In
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ARG22714 anti-CD25 antibody [KEI-alpha1] FACS image
Flow Cytometry: Rabbit peripheral blood lymphocytes stained with ARG22714 anti-CD25 antibody [KEI-alpha1] followed by Goat anti-Mouse IgG (FITC).
