ARG23009

anti-CD25 antibody [IL-A111] (FITC)

anti-CD25 antibody [IL-A111] (FITC) for Flow cytometry and Bovine,Sheep

Immune System antibody; Pre-B Cell Marker antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [IL-A111] recognizes CD25
Mouse anti Bovine CD25 antibody, clone IL-A111 recognizes the bovine CD25 cell surface antigen, a ~55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain. Bovine CD25 is expressed by activated T cells.Clone IL-A111 is reported to block the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes (Naessens et al. 1992).
Tested Reactivity Bov, Sheep
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone IL-A111
Isotype IgG1
Target Name CD25
Antigen Species Bovine
Conjugation FITC
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:100
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide and 1% BSA
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 281861 Bovine IL2RA

Swiss-port # P12342 Bovine Interleukin-2 receptor subunit alpha

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
Research Area Immune System antibody; Pre-B Cell Marker antibody
Calculated MW 31 kDa

Images (2) Click the Picture to Zoom In

  • ARG23009 anti-CD25 antibody [IL-A111] (FITC) FACS image

    Flow Cytometry: Figure A. ARG23009 anti-CD25 antibody [IL-A111] (FITC) and RPE conjugated mouse IgG2a isotype control. Figure B. ARG23009 anti-CD25 antibody [IL-A111] (FITC) and RPE conjugated mouse anti bovine CD4. All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

  • ARG23009 anti-CD25 antibody [IL-A111] (FITC) FACS image

    Flow Cytometry: Figure A. RPE conjugated mouse anti bovine CD4 and FITC conjugated mouse IgG1 isotype control. Figure B. RPE conjugated mouse anti bovine CD4 and ARG23009 anti-CD25 antibody [IL-A111] (FITC). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.