ARG23332

anti-CD25 antibody [B-B10] (low endotoxin)

anti-CD25 antibody [B-B10] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections and Human

Immune System antibody; Pre-B Cell Marker antibody

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [B-B10] recognizes CD25
Tested Reactivity Hu
Tested Application FACS, FuncSt, IHC-Fr
Specificity This antibody recognizes the Interleukin 2 Receptor a chain (IL-2 Ra), a 55 kDa protein.
Host Mouse
Clonality Monoclonal
Clone B-B10
Isotype IgG1
Target Name CD25
Antigen Species Human
Immunogen PHA activated T cells
Conjugation Un-conjugated
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
FuncStAssay-dependent
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note Sterile-filtered through 0.22 µm and treated to remove endotoxins.
Buffer PBS
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3559 Human IL2RA

Swiss-port # P01589 Human Interleukin-2 receptor subunit alpha

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
Research Area Immune System antibody; Pre-B Cell Marker antibody
Calculated MW 31 kDa

Images (1) Click the Picture to Zoom In

  • ARG23332 anti-CD25 antibody [B-B10] (low endotoxin) FACS image

    Flow Cytometry: PHA activated lymphocytes stained with ARG23332 anti-CD25 antibody [B-B10] (low endotoxin).