ARG20886
anti-CD25 antibody [7D4] (PE-Cyanine 7)
anti-CD25 antibody [7D4] (PE-Cyanine 7) for Blocking,Depletion,Flow cytometry,IHC-Frozen sections,Neutralizing and Mouse
Immune System antibody; Pre-B Cell Marker antibody
Overview
| Product Description | PE-Cyanine 7-conjugated Rat Monoclonal antibody [7D4] recognizes CD25 |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | BL, Depletion, FACS, IHC-Fr, Neut |
| Specificity | Mouse CD25. The clone 7D4 reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by The clone 3C7. While The clone 7D4 does not block the binding of IL-2 to CD25, when used in combination with The clone 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 7D4 |
| Isotype | IgM, kappa |
| Target Name | CD25 |
| Antigen Species | Mouse |
| Immunogen | IL-2 dependent BALB/c helper T cell clone HT2 |
| Conjugation | PE-Cyanine 7 |
| Alternate Names | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS, 0.1% Sodium azide and Sucrose. |
| Preservative | 0.1% Sodium azide |
| Stabilizer | Sucrose |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01590 Mouse Interleukin-2 receptor subunit alpha |
|---|---|
| Gene Symbol | IL2RA |
| Gene Full Name | interleukin 2 receptor, alpha chain |
| Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] |
| Function | Receptor for interleukin-2. [UniProt] |
| Research Area | Immune System antibody; Pre-B Cell Marker antibody |
| Calculated MW | 31 kDa |
Images (2) Click the Picture to Zoom In
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ARG20886 anti-CD25 antibody [7D4] (PE-Cyanine 7) FACS image
Flow Cytometry: Con-A-stimulated BALB/c Mouse splenocytes stained with ARG20886 anti-CD25 antibody [7D4] (PE-Cyanine 7).
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ARG20886 anti-CD25 antibody [7D4] (PE-Cyanine 7) FACS image
Flow Cytometry: C57BL/6 Mouse cytotoxic T lymphocyte cell line CTLL-2 was stained with ARG20886 anti-CD25 antibody [7D4] (PE-Cyanine 7).
