ARG56039
anti-CD25 antibody [143-13] (azide free)
anti-CD25 antibody [143-13] (azide free) for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections and Human
Immune System antibody; Pre-B Cell Marker antibody
Overview
| Product Description | Azide free Mouse Monoclonal antibody [143-13] recognizes CD25 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr |
| Specificity | The mAb [143-13] recognizes the epitope region B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 143-13 |
| Isotype | IgG1, kappa |
| Target Name | CD25 |
| Antigen Species | Human |
| Immunogen | Stimulated Human leukocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01589 Human Interleukin-2 receptor subunit alpha |
|---|---|
| Gene Symbol | IL2RA |
| Gene Full Name | interleukin 2 receptor, alpha |
| Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] |
| Function | Receptor for interleukin-2. [UniProt] |
| Cellular Localization | Cell surface |
| Research Area | Immune System antibody; Pre-B Cell Marker antibody |
| Calculated MW | 31 kDa |
