ARG23152
anti-CD244 antibody [2B4.69]
anti-CD244 antibody [2B4.69] for Flow cytometry,Immunoprecipitation and Human
Overview
| Product Description | Mouse Monoclonal antibody [2B4.69] recognizes CD244 Mouse anti Human CD244 antibody, clone 2B4.69 recognizes the CD244 cell surface antigen, also known as natural killer receptor 2B4 (NKG2B4). CD244 is a 370 amino acid single pass type I transmembrane glycoprotein with two Ig-like V type domains. CD244 is a member of the SLAM family of receptors which is expressed on natural killer (NK) cells, gamma delta T cells, CD14+ monocytes, and a subset of CD8+ T cells.CD244 was originally identified as an activating receptor on NK cells, although recent studies suggest that the receptor may have a wide range of activities including NK cell activation, costimulation, or even inhibition, depending on the cell type and stage of differentiation. The ligand of CD244 is CD48, a GPI-anchored membrane protein also belonging to the SLAM family. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2B4.69 |
| Isotype | IgG1 |
| Target Name | CD244 |
| Antigen Species | Human |
| Immunogen | CD244 Fusion protein. |
| Conjugation | Un-conjugated |
| Alternate Names | NK cell activation-inducing ligand; h2B4; Natural killer cell receptor 2B4; CD antigen CD244; 2B4; NAIL; NKR2B4; SLAM family member 4; Nmrk; SLAMF4; Signaling lymphocytic activation molecule 4; NK cell type I receptor protein 2B4 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD244 |
| Gene Full Name | CD244 molecule, natural killer cell receptor 2B4 |
| Background | This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
| Function | Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family. [UniProt] |
| Calculated MW | 42 kDa |
| PTM | N-linked glycosylation is essential for the binding to its ligand CD48. Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding. Phosphorylated by FYN and CSK on tyrosine residues following activation. Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells. [UniProt] |
