ARG23142
anti-CD238 / KELL antibody [BRIC203]
anti-CD238 / KELL antibody [BRIC203] for Flow cytometry,Immunoprecipitation and Human
Overview
| Product Description | Mouse Monoclonal antibody [BRIC203] recognizes CD238 / KELL Mouse anti Human CD238 antibody, clone BRIC203 recognizes human CD238, also known as the kell blood group antigen Kpbc. The kell blood group antigens are carried on the ~93 kDa erythrocyte membrane glycoprotein.In hemagglutination tests, Mouse anti Human CD238 antibody, clone BRIC203 reacts with Kp (a-b+) but not Kp (a+b-). In addition Mouse anti Human CD238 antibody, clone BRIC203 will agglutinate Kp (a+b-c+) and Kp (a-b-c+) erythrocytes but not react with Ko erythrocytes. Weaker staining in flow cytometry is observed on erythroblasts derived from CD34+ hematopoietic progenitor cells, compared to Kp (a-b+) red blood cells. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BRIC203 |
| Isotype | IgG1 |
| Target Name | CD238 / KELL |
| Antigen Species | Human |
| Immunogen | Human erythrocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | CD238; Kell blood group glycoprotein; ECE3; CD antigen CD238; EC 3.4.24.- |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KEL |
| Gene Full Name | Kell blood group, metallo-endopeptidase |
| Background | This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases. [provided by RefSeq, Jul 2008] |
| Function | Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3, with a marked preference for EDN3. [UniProt] |
| Calculated MW | 83 kDa |
| PTM | N-glycosylated. [UniProt] |
