ARG53798
anti-CD230 / Prion protein antibody [EM-20]
anti-CD230 / Prion protein antibody [EM-20] for Western blot and Human
Developmental Biology antibody; Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [EM-20] recognizes CD230 / Prion protein |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | The clone EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | EM-20 |
| Isotype | IgG2a |
| Target Name | CD230 / Prion protein |
| Antigen Species | Human |
| Immunogen | Recombinant human prion protein |
| Conjugation | Un-conjugated |
| Alternate Names | GSS; PrPc; PrP33-35C; PrP27-30; Alternative prion protein; p27-30; CJD; ASCR; CD230; PrP; PRIP; KURU; AltPrP |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Non-reducing condition are essential. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRNP |
| Gene Full Name | prion protein |
| Background | CD230 / Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K. |
| Research Area | Developmental Biology antibody; Neuroscience antibody |
| Calculated MW | 9 kDa |
| PTM | The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion. Isoform 2 is sumoylated with SUMO1. |
Images (1) Click the Picture to Zoom In
-
ARG53798 anti-CD230 / Prion protein antibody [EM-20] WB image
Western blot: 1,2. Creutzfeld-Jakob disease (CJD) negative Human brain tissue lysate 3,4. CJD positive Human brain tissue lysate stained with ARG53798 anti-CD230 / Prion protein antibody [EM-20].
CJD positive patient has proteinase K resistent prion protein. Lane 1,4: Samples with proteinase K treatment. Lane 2,3: Samples without proteinase K treatment.
