ARG23154
anti-CD230 / Prion protein antibody [2G11]
anti-CD230 / Prion protein antibody [2G11] for ELISA,IHC-Formalin-fixed paraffin-embedded sections and Sheep
Overview
| Product Description | Mouse Monoclonal antibody [2G11] recognizes CD230 / Prion protein |
|---|---|
| Tested Reactivity | Sheep |
| Tested Application | ELISA, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2G11 |
| Isotype | IgG2a |
| Target Name | CD230 / Prion protein |
| Antigen Species | Sheep |
| Immunogen | Synthetic peptide 146-R154R171-182 of Sheep PrP. |
| Conjugation | Un-conjugated |
| Alternate Names | GSS; PrPc; PrP33-35C; PrP27-30; Alternative prion protein; p27-30; CJD; ASCR; CD230; PrP; PRIP; KURU; AltPrP |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Treatment of tissue sections in 98% formic acid, for 30 minutes, is recommended prior to pre-treatment with trypsin at 37°C for 5 minutes followed by heat mediated retrieval with 10mM Citrate buffer (pH 6.0). ELISA: Tested on peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | PRNP |
|---|---|
| Gene Full Name | prion protein |
| Background | The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
| Calculated MW | 9 kDa |
| PTM | The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion. Isoform 2 is sumoylated with SUMO1. [UniProt] |
