ARG42259
anti-CD229 antibody [HLy9.25] (low endotoxin)
anti-CD229 antibody [HLy9.25] (low endotoxin) for Flow cytometry,Functional study,ICC/IF,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [HLy9.25] recognizes CD229 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, ICC/IF, IP, WB |
| Specificity | The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HLy9.25 |
| Isotype | IgG1 |
| Target Name | CD229 |
| Antigen Species | Human |
| Immunogen | CD229-transfected 300.19 pre-B cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | Signaling lymphocytic activation molecule 3; T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; hly9; Lymphocyte antigen 9; mLY9; CD229; SLAMF3; CD antigen CD229; SLAM family member 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LY9 |
| Gene Full Name | lymphocyte antigen 9 |
| Background | LY9 belongs to the SLAM family of immunomodulatory receptors (see SLAMF1; MIM 603492) and interacts with the adaptor molecule SAP (SH2D1A; MIM 300490) (Graham et al., 2006 [PubMed 16365421]).[supplied by OMIM, Mar 2008] |
| Function | Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A (PubMed:22184727). Promotes recruitment of RORC to the IL-17 promoter (PubMed:22989874). May be involved in the maintenance of peripheral cell tolerance by serving as a negative regulator of the immune response. May disable autoantibody responses and inhibit IFN-gamma secretion by CD4(+) T-cells. May negatively regulate the size of thymic innate CD8(+) T-cells and the development of invariant natural killer T (iNKT) cells (By similarity). [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Cell membrane. [UniProt] |
| Calculated MW | 72 kDa |
Images (2) Click the Picture to Zoom In
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ARG42259 anti-CD229 antibody [HLy9.25] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG42259 anti-CD229 antibody [HLy9.25] (low endotoxin) at 16 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG42259 anti-CD229 antibody [HLy9.25] (low endotoxin) FACS image
Flow Cytometry: Separation of Human CD229 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG42259 anti-CD229 antibody [HLy9.25] (low endotoxin) at 16 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
