ARG58760
anti-CD223 / LAG3 antibody
anti-CD223 / LAG3 antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes CD223 / LAG3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD223 / LAG3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human CD223 / LAG3 (NP_002277.4). (EDVSQAQAGTYTCH) |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD223; CD223; Protein FDC; Lymphocyte activation gene 3 protein; LAG-3 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P18627 Human Lymphocyte activation gene 3 protein |
|---|---|
| Gene Symbol | LAG3 |
| Gene Full Name | lymphocyte-activation gene 3 |
| Background | Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008] |
| Function | Involved in lymphocyte activation. Binds to HLA class-II antigens. [UniProt] |
| Calculated MW | 57 kDa |
Images (1) Click the Picture to Zoom In
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ARG58760 anti-CD223 / LAG3 antibody WB image
Western blot: 35 µg of Human lymph node and Human tonsil lysates (in RIPA buffer) stained with ARG58760 anti-CD223 / LAG3 antibody at 1 µg/ml and 0.5 µg/ml respectively. Primary incubation was 1 hour. Detected by chemiluminescence.
