ARG58749
anti-CD223 / LAG3 antibody [2G8]
anti-CD223 / LAG3 antibody [2G8] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal [2G8] antibody recognizes CD223 / LAG3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2G8 |
| Isotype | IgG1, kappa |
| Target Name | CD223 / LAG3 |
| Antigen Species | Human |
| Immunogen | Extracellular domain of Human CD223 / LAG3. |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD223; CD223; Protein FDC; Lymphocyte activation gene 3 protein; LAG-3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Human lymphoma tissue and spleen tissue. | ||||||||
| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P18627 Human Lymphocyte activation gene 3 protein |
|---|---|
| Gene Symbol | LAG3 |
| Gene Full Name | lymphocyte-activation gene 3 |
| Background | Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008] |
| Function | Involved in lymphocyte activation. Binds to HLA class-II antigens. [UniProt] |
| Calculated MW | 57 kDa |
Images (4) Click the Picture to Zoom In
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ARG58749 anti-CD223 / LAG3 antibody [2G8] IF image
Immunofluorescence: LAG3 over expressing HEK293 cells stained with ARG58749 anti-CD223 / LAG3 antibody [2G8] at 2 µg/ml dilution.
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ARG58749 anti-CD223 / LAG3 antibody [2G8] FACS image
Flow Cytometry: LAG3 over expressing HEK293 cells stained with ARG58749 anti-CD223 / LAG3 antibody [2G8] at 1 µg/ml dilution. Blue: Untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
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ARG58749 anti-CD223 / LAG3 antibody [2G8] WB image
Western blot: LAG3 over expressing HEK293 cells stained with ARG58749 anti-CD223 / LAG3 antibody [2G8] at 0.25, 0.5 and 1 µg/ml dilution.
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ARG58749 anti-CD223 / LAG3 antibody [2G8] ICC image
Immunocytochemistry: LAG3 over expressing HEK293 cells stained with ARG58749 anti-CD223 / LAG3 antibody [2G8]. Lower left (control): Mouse IgG antibody at 1 µg/ml dilution.
