ARG62777
anti-CD222 / IGF2R antibody [MEM-238]
anti-CD222 / IGF2R antibody [MEM-238] for Flow cytometry,Western blot and Human,Primates
Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-238] recognizes CD222 / IGF2R |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS, WB |
| Specificity | The clone MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-238 |
| Isotype | IgG1 |
| Target Name | CD222 / IGF2R |
| Immunogen | Recombinant Vaccinia virus encoding CD222. |
| Conjugation | Un-conjugated |
| Alternate Names | CD222; MPR 300; Insulin-like growth factor II receptor; M6P/IGF2R; MPRI; 300 kDa mannose 6-phosphate receptor; IGF-II receptor; CI Man-6-P receptor; M6P/IGF2 receptor; MPR1; CIMPR; Cation-independent mannose-6-phosphate receptor; CD antigen CD222; Insulin-like growth factor 2 receptor; M6P-R; CI-MPR; M6PR |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11717 Human Cation-independent mannose-6-phosphate receptor |
|---|---|
| Gene Symbol | IGF2R |
| Gene Full Name | insulin-like growth factor 2 receptor |
| Background | CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria. |
| Function | Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. [UniProt] |
| Research Area | Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 274 kDa |
Images (1) Click the Picture to Zoom In
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ARG62777 anti-CD222 / IGF2R antibody [MEM-238] WB image
Western blot: Jurkat cell lysate stained with 1) ARG62777 anti-CD222 / IGF2R antibody [MEM-238], or 2) Isotype Mouse IgG1 control antibody.
Clone References
Alix serves as an adaptor that allows human parainfluenza virus type 1 to interact with the host cell ESCRT system.
ICC/IF / Human
The C proteins of human parainfluenza virus type 1 block IFN signaling by binding and retaining Stat1 in perinuclear aggregates at the late endosome.
ICC/IF /
Histidine-rich glycoprotein binds heparanase and regulates its enzymatic activity and cell surface interactions.
FACS / Human
The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport.
ICC/IF / Human
Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression.
Inhib / Human
Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation.
IP / Mouse
