ARG23476
anti-CD21 antibody [CC51]
anti-CD21 antibody [CC51] for Flow cytometry and Bovine,Pig
Overview
| Product Description | Mouse Monoclonal antibody [CC51] recognizes CD21 Mouse anti Bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessens et al. 1990). CD21 may be expressed on B-cells as either a long or a short form (Pringle et al. 2012). Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkora et. al. 2013). |
|---|---|
| Tested Reactivity | Bov, Pig |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CC51 |
| Isotype | IgG2b |
| Target Name | CD21 |
| Antigen Species | Bovine |
| Immunogen | Bovine (Friesian cattle) mesenteric lymph node cells |
| Conjugation | Un-conjugated |
| Alternate Names | Cr2; Complement C3d receptor; C3DR; CD21; CD antigen CD21; Complement receptor type 2; SLEB9; CR; CVID7; Epstein-Barr virus receptor; EBV receptor |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CR2 |
|---|---|
| Gene Full Name | complement component (3d/Epstein Barr virus) receptor 2 |
| Background | This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| Function | Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. [UniProt] |
| Calculated MW | 113 kDa |
Images (1) Click the Picture to Zoom In
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ARG23476 anti-CD21 antibody [CC51] FACS image
Flow Cytometry: Bovine peripheral blood lymphocytes stained with ARG23476 anti-CD21 antibody [CC51] followed by Goat anti Mouse IgG (FITC).
