ARG65456
anti-CD205 antibody [HD30]
anti-CD205 antibody [HD30] for Flow cytometry,IHC-Frozen sections,Western blot and Human
Controls and Markers antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [HD30] recognizes CD205 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-Fr, WB |
| Specificity | The clone HD30 recognizes CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HD30 |
| Isotype | IgG1 |
| Target Name | CD205 |
| Antigen Species | Human |
| Immunogen | Recombinant Fc-tagged human CD205 |
| Conjugation | Un-conjugated |
| Alternate Names | Lymphocyte antigen 75; DEC-205; DEC205; Ly-75; CD205; CD antigen CD205 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Only non-denatured samples. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LY75 |
| Gene Full Name | lymphocyte antigen 75 |
| Background | CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells. |
| Function | Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes. [UniProt] |
| Research Area | Controls and Markers antibody; Immune System antibody |
| Calculated MW | 198 kDa |
Clone References
Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals.
