ARG22423

anti-CD204 / MSR1 antibody [2F8] (low endotoxin)

anti-CD204 / MSR1 antibody [2F8] (low endotoxin) for ELISA,Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Mouse,Catfish,Pig

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [2F8] recognizes CD204 / MSR1
This antibody recognizes the murine scavenger receptor class A (SRA), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993). Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).
Tested Reactivity Ms, Cfsh, Pig
Tested Application ELISA, FACS, FuncSt, IHC-Fr, IP, WB
Host Rat
Clonality Monoclonal
Clone 2F8
Isotype IgG2b
Target Name CD204 / MSR1
Antigen Species Mouse
Immunogen Raw 264 cell line.
Conjugation Un-conjugated
Alternate Names Macrophage scavenger receptor types I and II; SR-A; SCARA1; Macrophage acetylated LDL receptor I and II; SRA; CD antigen CD204; Scavenger receptor class A member 1; CD204; phSR1; phSR2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACSNeat
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
WB: This product recognises CD204 in J774 cells under non-reduced conditions only.
FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note Low endotoxin
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20288 Mouse MSR1

Swiss-port # P30204 Mouse Macrophage scavenger receptor types I and II

Gene Symbol Msr1
Gene Full Name macrophage scavenger receptor 1
Background This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]
Function Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize acetylated LDL. [UniProt]
Calculated MW 50 kDa

Images (2) Click the Picture to Zoom In

  • ARG22423 anti-CD204 / MSR1 antibody [2F8] (low endotoxin) FACS image

    Flow Cytometry: Mouse peritoneal macrophages stained with ARG22423 anti-CD204 / MSR1 antibody [2F8] (low endotoxin).

  • ARG22423 anti-CD204 / MSR1 antibody [2F8] (low endotoxin) WB image

    Western blot: J774 cell lysate (non reduced) stained with ARG22423 anti-CD204 / MSR1 antibody [2F8] (low endotoxin).