ARG22652
anti-CD203a antibody [PM18-7]
anti-CD203a antibody [PM18-7] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Pig
Overview
| Product Description | Mouse Monoclonal antibody [PM18-7] recognizes CD203a This antibody recognizes Porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguez et al. 1998) and later identified as the Porcine homologue of Human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or ENPP1 (Petersen et al. 2007). Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 kDa and one of ~130 kDa (Dominguez et al. 1998) under both reducing and non-reducing conditions. Subsequent studies suggest that CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersen et al. 2007) from preparations of Porcine alveolar macrophages. CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersen et al. 2007, Hwang et al. 2015), it is not expressed on monocyte populations (McCullough et al. 1997, Hwang et al. 2015). SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999). |
|---|---|
| Tested Reactivity | Pig |
| Tested Application | FACS, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PM18-7 |
| Isotype | IgG1 |
| Target Name | CD203a |
| Antigen Species | Pig |
| Immunogen | Porcine alveolar macrophages. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (1) Click the Picture to Zoom In
-
ARG22652 anti-CD203a antibody [PM18-7] FACS image
Flow Cytometry: Pig alveolar macrophages stained with ARG22652 anti-CD203a antibody [PM18-7] followed by Goat anti-Mouse IgG (FITC).
