ARG62403
anti-CD2 antibody [B336 (HuLy-m1)]
anti-CD2 antibody [B336 (HuLy-m1)] for Flow cytometry,IHC-Frozen sections and Human
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [B336 (HuLy-m1)] recognizes CD2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-Fr |
| Specificity | Reacts with the majority of T-cells in peripheral lymphoid tissue, cortical thymocytes, and most T-cell neoplasms. Also reacts with the E-rosette receptor on T-cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B336 (HuLy-m1) |
| Isotype | IgG2b |
| Target Name | CD2 |
| Antigen Species | Human |
| Immunogen | Human thymocytes (3 pooled donors) |
| Conjugation | Un-conjugated |
| Alternate Names | T-cell surface antigen T11/Leu-5; LFA-3 receptor; T-cell surface antigen CD2; SRBC; Erythrocyte receptor; CD antigen CD2; T11; Rosette receptor; LFA-2 |
Application Instructions
| Application Note | FACS: 1-5 mg/106 cells. IHC-Fr: 10-20 ug/mL. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Buffer | 1X PBS buffer with <0.1% sodium azide. |
| Preservative | <0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD2 |
| Gene Full Name | CD2 molecule |
| Background | CD2 is a 50 kDa single-chain type I transmembrane glycoprotein, originally defined as the sheep erythrocyte receptor. CD2 is one of the earliest T-cell lineage-restricted antigens to appear during T-cell differentiation, and it is expressed on most thymocytes, peripheral T cells and NK cells. Originally identified as a sheep RBC receptor, functions as a CD58 receptor and mediates activation of T-cells, NK cells and dendritic cells. |
| Function | CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 39 kDa |
