ARG62403

anti-CD2 antibody [B336 (HuLy-m1)]

anti-CD2 antibody [B336 (HuLy-m1)] for Flow cytometry,IHC-Frozen sections and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [B336 (HuLy-m1)] recognizes CD2
Tested Reactivity Hu
Tested Application FACS, IHC-Fr
Specificity Reacts with the majority of T-cells in peripheral lymphoid tissue, cortical thymocytes, and most T-cell neoplasms. Also reacts with the E-rosette receptor on T-cells.
Host Mouse
Clonality Monoclonal
Clone B336 (HuLy-m1)
Isotype IgG2b
Target Name CD2
Antigen Species Human
Immunogen Human thymocytes (3 pooled donors)
Conjugation Un-conjugated
Alternate Names T-cell surface antigen T11/Leu-5; LFA-3 receptor; T-cell surface antigen CD2; SRBC; Erythrocyte receptor; CD antigen CD2; T11; Rosette receptor; LFA-2

Application Instructions

Application Note FACS: 1-5 mg/106 cells.
IHC-Fr: 10-20 ug/mL.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 1X PBS buffer with <0.1% sodium azide.
Preservative <0.1% sodium azide.
Concentration 2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 914 Human CD2

Swiss-port # P06729 Human T-cell surface antigen CD2

Gene Symbol CD2
Gene Full Name CD2 molecule
Background CD2 is a 50 kDa single-chain type I transmembrane glycoprotein, originally defined as the sheep erythrocyte receptor. CD2 is one of the earliest T-cell lineage-restricted antigens to appear during T-cell differentiation, and it is expressed on most thymocytes, peripheral T cells and NK cells. Originally identified as a sheep RBC receptor, functions as a CD58 receptor and mediates activation of T-cells, NK cells and dendritic cells.
Function CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 39 kDa