ARG65539

anti-CD1b antibody [SN13]

anti-CD1b antibody [SN13] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [SN13] recognizes CD1b
Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, IHC-Fr, IHC-P, IP
Specificity The clone SN13 (also known as K5-1B8) recognizes CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.
Host Mouse
Clonality Monoclonal
Clone SN13
Isotype IgG1
Target Name CD1b
Antigen Species Human
Immunogen A cell membrane antigen preparation that was isolated from normal human thymocytes
Conjugation Un-conjugated
Alternate Names T-cell surface glycoprotein CD1b; CD1A; R1; CD antigen CD1b; CD1

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 910 Human CD1B

Swiss-port # P29016 Human T-cell surface glycoprotein CD1b

Gene Symbol CD1B
Gene Full Name CD1b molecule
Background CD1b (also known as R1) together with CD1a and c, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. Besides non-peptide glycolipid antigen presentation to CD1-restricted T cells, CD1b has been implicated in thymocyte development.
Function Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. [UniProt]
Highlight Related products:
CD1b antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Immune System antibody
Calculated MW 37 kDa

Clone References

Id1 and NF-κB promote the generation of CD133+ and BMI-1+ keratinocytes and the growth of xenograft tumors in mice.

WB / Human

Lai J et al.
Int J Oncol.,  (2014)

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Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells.

Giuliani A et al.
Infect Immun.,  (2001)

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