ARG65392
anti-CD197 / CCR7 antibody [4B12]
anti-CD197 / CCR7 antibody [4B12] for CyTOF®-candidate,Flow cytometry,Immunoprecipitation and Mouse
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Rat Monoclonal antibody [4B12] recognizes CD197 / CCR7 |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | CyTOF®-candidate, FACS, IP |
| Specificity | The rat monoclonal antibody 4B12 recognizes murine CD197 / CCR7 (chemokine receptor 7), a 43 kDa Gproteincoupled receptor for chemokines CCL19 and CCL21. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 4B12 |
| Isotype | IgG2a |
| Target Name | CD197 / CCR7 |
| Antigen Species | Mouse |
| Immunogen | Murine CD197-transfected RBL-2H3 cells |
| Conjugation | Un-conjugated |
| Alternate Names | C-C chemokine receptor type 7; CD197; CD antigen CD197; CCR-7; CC-CKR-7; BLR2; CMKBR7; C-C CKR-7; MIP-3 beta receptor; EBV-induced G-protein coupled receptor 1; EBI1; Epstein-Barr virus-induced G-protein coupled receptor 1; CDw197 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-G affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Ccr7 |
| Gene Full Name | chemokine (C-C motif) receptor 7 |
| Background | CD197 / CCR7 (chemokine receptor 7) is a seven membrane-spanning protein serving as a receptor for chemokines CCL19 and CCL21. It is expressed on most naive T cells, some hematopoietic stem cells, mature dendritic cells, NK cells and some memory T cells and B cells subsets. CD197 plays important roles in development of thymocytes, and regulating the recirculation and homing of lymphocytes and dendritic cells to secondary lymphoid organs. |
| Function | Receptor for the MIP-3-beta chemokine. [UniProt] |
| Highlight | Related products: CD197 antibodies; Anti-Rat IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 43 kDa |
Images (1) Click the Picture to Zoom In
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ARG65392 anti-CD197 / CCR7 antibody [4B12] FACS image
Flow Cytometry: Unstained (blue) and stained (red) Murine splenocytes. Cells were stained with ARG65392 anti-CD197 / CCR7 antibody [4B12] at 4 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
Clone References
The autoimmune diabetes locus Idd9 regulates development of type 1 diabetes by affecting the homing of islet-specific T cells.
CCR7 governs skin dendritic cell migration under inflammatory and steady-state conditions.
Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.
