ARG62759
anti-CD19 antibody [4G7]
anti-CD19 antibody [4G7] for Flow cytometry,ICC/IF and Human
Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; B cell Marker antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody; Immature B Cell Marker antibody; Follicular dendritic cells antibody
Overview
| Product Description | Mouse Monoclonal antibody [4G7] recognizes CD19 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF |
| Specificity | The clone 4G7 recognizes CD19 (B4), a 95 kDa type I transmembrane glycoprotein of immunoglobulin superfamily, expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells. WS Code: 2 B43 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4G7 |
| Isotype | IgG1 |
| Target Name | CD19 |
| Antigen Species | Human |
| Immunogen | Human CCL (chronic lymphocytic leukemia) cells |
| Conjugation | Un-conjugated |
| Alternate Names | Differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD antigen CD19; CVID3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD19 |
| Gene Full Name | CD19 molecule |
| Background | CD19: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008] |
| Function | CD19 functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701). [UniProt] |
| Highlight | Related products: CD19 antibodies; CD19 ELISA Kits; CD19 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; B cell Marker antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody; Immature B Cell Marker antibody; Follicular dendritic cells antibody |
| Calculated MW | 61 kDa |
| PTM | Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. |
Images (2) Click the Picture to Zoom In
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ARG62759 anti-CD19 antibody [4G7] FACS image
Flow Cytometry: Human peripheral blood stained with ARG62759 anti-CD19 antibody [4G7] at 3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62759 anti-CD19 antibody [4G7] FACS image
Flow Cytometry: Separation of human CD19 positive lymphocytes (red-filled) from CD19 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62759 anti-CD19 antibody [4G7] at 3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
