ARG62752
anti-CD18 / LFA1 beta antibody [MEM-148]
anti-CD18 / LFA1 beta antibody [MEM-148] for CyTOF®-candidate,Flow cytometry,Functional study,Immunoprecipitation,Western blot and Human
Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
Product Description | Mouse Monoclonal antibody [MEM-148] recognizes CD18 / LFA1 beta |
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Tested Reactivity | Hu |
Species Does Not React With | Pig |
Tested Application | CyTOF®-candidate, FACS, FuncSt, IP, WB |
Specificity | The clone MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit; beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. HLDA VI; WS Code AS A052 |
Host | Mouse |
Clonality | Monoclonal |
Clone | MEM-148 |
Isotype | IgG1 |
Target Name | CD18 / LFA1 beta |
Immunogen | Peripheral blood mononuclear cells |
Conjugation | Un-conjugated |
Alternate Names | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
Application Instructions
Application Suggestion |
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Application Note | FACS: The clone MEM-148 is an excellent marker of activated myeloid cells. WB: Under non-reducing condition. Functional studies: The clone MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
Preservative | 15 mM Sodium azide |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | ITGB2 |
Gene Full Name | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
Background | CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. |
Function | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt] |
Highlight | Related products: CD18 antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
Research Area | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
Calculated MW | 85 kDa |
PTM | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
Images (2) Click the Picture to Zoom In
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ARG62752 anti-CD18 / LFA1 beta antibody [MEM-148] WB image
Western blot: PMA-activated neutrophils stained with ARG62752 anti-CD18 / LFA1 beta antibody [MEM-148].
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ARG62752 anti-CD18 / LFA1 beta antibody [MEM-148] WB image
Western blot: PMA-activated monocytes stained with ARG62752 anti-CD18 / LFA1 beta antibody [MEM-148].
Clone References