ARG62757

anti-CD18 / LFA1 beta antibody [M18 / 2]

anti-CD18 / LFA1 beta antibody [M18 / 2] for Flow cytometry,Functional study,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse

Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Rat Monoclonal antibody [M18/2] recognizes CD18 / LFA1 beta
Tested Reactivity Ms
Species Does Not React With Dog
Tested Application FACS, FuncSt, IHC-Fr, IHC-P, IP, WB
Specificity The clone M18/2 recognizes CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
Host Rat
Clonality Monoclonal
Clone M18/2
Isotype IgG2a
Target Name CD18 / LFA1 beta
Antigen Species Mouse
Immunogen Murine cytotoxic T cell glycoproteins
Conjugation Un-conjugated
Alternate Names MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note Functional studies: Blocking or stimulation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by protein A
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16414 Mouse ITGB2

Swiss-port # P11835 Mouse Integrin beta-2

Gene Symbol Itgb2
Gene Full Name integrin beta 2
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts.
Function Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 85 kDa
PTM Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).

Images (2) Click the Picture to Zoom In

  • ARG62757 anti-CD18 / LFA1 beta antibody [M18 / 2] FACS image

    Flow Cytometry: Murine splenocytes stained with ARG62757 anti-CD18 / LFA1 beta antibody [M18 / 2] at 16 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.

  • ARG62757 anti-CD18 / LFA1 beta antibody [M18 / 2] FACS image

    Flow Cytometry: Separation of unstained (black-dashed) and stained (red-filled) murine myeloid cells from murine splenocyte suspension. Cells were stained with ARG62757 anti-CD18 / LFA1 beta antibody [M18 / 2] at 16 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.

Clone References

Neutrophil-selective CD18 silencing using RNA interference in vivo.

Cullere X et al.
Blood.,  (2008)

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Manifestations of inflammatory arthritis are critically dependent on LFA-1.

Watts GM et al.
J Immunol.,  (2005)

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CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes.

Barlow SC et al.
Am J Pathol.,  (2004)

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Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization.

Sakurai E et al.
Invest Ophthalmol Vis Sci.,  (2003)

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Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1.

Abraham C et al.
J Immunol.,  (2001)

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