ARG62754
anti-CD18 / LFA1 beta antibody [IVA35]
anti-CD18 / LFA1 beta antibody [IVA35] for Flow cytometry,ICC/IF,Immunoprecipitation and Bovine
Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [IVA35] recognizes CD18 / LFA1 beta |
|---|---|
| Tested Reactivity | Bov |
| Tested Application | FACS, ICC/IF, IP |
| Specificity | The clone IVA35 reacts with CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | IVA35 |
| Isotype | IgG1 |
| Target Name | CD18 / LFA1 beta |
| Immunogen | Bovine leukocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGB2 |
| Gene Full Name | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| Background | This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Function | Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (By similarity). Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 85 kDa |
| PTM | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
Images (3) Click the Picture to Zoom In
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ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] ICC/IF image
Immunofluorescence: Acetone / methanol-fixed Bovine peripheral blood (lymphocyte-enriched suspension) stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] (green), DAPI (blue) for nuclear staining.
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ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image
Flow Cytometry: Separation of stained bovine lymphocytes (red-filled) from unstained bovine lymphocytes (black-dashed). Cells were stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image
Flow Cytometry: Bovine peripheral whole blood stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
Monoclonal antibodies specific for bovine CD18.
Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995.
