ARG23941
anti-CD18 / LFA1 beta antibody [BAQ30A]
anti-CD18 / LFA1 beta antibody [BAQ30A] for Flow cytometry and Human,Bovine,Cat,Dog,Goat,Sheep
Overview
| Product Description | Mouse Monoclonal antibody [BAQ30A] recognizes CD18 / LFA1 beta |
|---|---|
| Tested Reactivity | Hu, Bov, Cat, Dog, Goat, Sheep |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BAQ30A |
| Isotype | IgG1 |
| Target Name | CD18 / LFA1 beta |
| Immunogen | Cells from multiple species. |
| Conjugation | Un-conjugated |
| Alternate Names | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Unpurified |
| Purification Note | 0.2 µm filtered. |
| Buffer | Tissue culture supernatant and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGB2 |
| Gene Full Name | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| Background | This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Function | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 85 kDa |
| PTM | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG23941 anti-CD18 / LFA1 beta antibody [BAQ30A] FACS image
Flow Cytometry: Bovine peripheral blood lymphocytes stained with ARG23941 anti-CD18 / LFA1 beta antibody [BAQ30A] at 10 µg/ml followed by Goat anti-Mouse IgG1 (PE) antibody. The antibody was plotted against Bovine CD4 as recognized by ARG23425 anti-CD4 antibody [CC8] (FITC).
