ARG52704
anti-CD16a antibody [SP189]
anti-CD16a antibody [SP189] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
Overview
| Product Description | Rabbit Monoclonal antibody [SP189] recognizes CD16a |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP189 |
| Isotype | IgG |
| Target Name | CD16a |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from the C-terminus of human CD16a protein. |
| Conjugation | Un-conjugated |
| Alternate Names | FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Lung, Tonsil, K562 Cell Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08637 Human Low affinity immunoglobulin gamma Fc region receptor III-A |
|---|---|
| Background | CD16 is a cluster of differentiation found on the surface of natural killer or polymorphonuclear leukocytes (PMN) cells, neutrophils, monocytes and macrophages. There are two CD16 isoform CD16a and CD16b. CD16a is expressed in monocytes, macrophages and NK cells and CD16b is expressed in neutrophils. |
| Cellular Localization | Membrane |
| Highlight | Related products: CD16 antibodies; CD16 ELISA Kits; CD16 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody |
| Calculated MW | 29 kDa |
| PTM | Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms. The soluble form is produced by a proteolytic cleavage. |
Images (12) Click the Picture to Zoom In
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ARG52704 anti-CD16a antibody [SP189] WB image
Western Blot: K562 Cell Lysate stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human Lung stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human Spleen stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human Stomach Adenocarcinoma stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human Liver stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human Thymus stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human Liver Hepatocellular Carcinoma stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human Placenta stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human B Cell Lymphoma stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human Cervical Squamous Cell Carcinoma stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human Colon Adenocarcinoma stained with CD16a antibody [SP189] (ARG52704)
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ARG52704 anti-CD16a antibody [SP189] IHC-P image
Immunohistochemistry: Human HK Lymphoma stained with CD16a antibody [SP189] (ARG52704)
