ARG23465
anti-CD169 / Siglec 1 antibody [ED3]
anti-CD169 / Siglec 1 antibody [ED3] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Rat
Overview
| Product Description | Mouse Monoclonal antibody [ED3] recognizes CD169 / Siglec 1 |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | FACS, ICC/IF, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ED3 |
| Isotype | IgG2a |
| Target Name | CD169 / Siglec 1 |
| Antigen Species | Rat |
| Immunogen | Rat Spleen cell homogenate |
| Conjugation | Un-conjugated |
| Alternate Names | CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | SIGLEC1 |
|---|---|
| Gene Full Name | sialic acid binding Ig-like lectin 1, sialoadhesin |
| Background | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt] |
| Calculated MW | 183 kDa |
Images (3) Click the Picture to Zoom In
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ARG23465 anti-CD169 / Siglec 1 antibody [ED3] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23465 anti-CD169 / Siglec 1 antibody [ED3], red in A and Mouse anti Rat CD4, clone W3/25, green in B. C is the merged image with nuclei counter-staind blue using DAPI. (Low power).
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ARG23465 anti-CD169 / Siglec 1 antibody [ED3] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23465 anti-CD169 / Siglec 1 antibody [ED3], red in A and Mouse anti Rat CD4, clone W3/25, green in B. C is the merged image with nuclei counter-staind blue using DAPI. (Medium power).
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ARG23465 anti-CD169 / Siglec 1 antibody [ED3] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23465 anti-CD169 / Siglec 1 antibody [ED3], red in A and Mouse anti Rat CD4, clone W3/25, green in B. C is the merged image with nuclei counter-staind blue using DAPI. (High power).
