ARG65404
anti-CD169 / Siglec 1 antibody [7-239]
anti-CD169 / Siglec 1 antibody [7-239] for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
Immune System antibody; Microbiology and Infectious Disease antibody
Overview
| Product Description | Mouse Monoclonal antibody [7-239] recognizes CD169 / Siglec 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IHC-Fr, IP, WB |
| Specificity | The mouse monoclonal antibody 7239 recognizes CD169 (sialoadhesin, Siglec1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7-239 |
| Isotype | IgG1 |
| Target Name | CD169 / Siglec 1 |
| Antigen Species | Human |
| Immunogen | human rhinovirus 14-infected monocyte-derived dendritic cells |
| Conjugation | Un-conjugated |
| Alternate Names | CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: Inhibition of erythrocyte rosetting with cells expressing CD169. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SIGLEC1 |
| Gene Full Name | sialic acid binding Ig-like lectin 1, sialoadhesin |
| Background | CD169, also known as Siglec-1 or sialoadhesin, is a type I transmembrane glycoprotein of the sialic acid binding Ig-like lectin family. It binds to sialylated glycoproteins on various haematopoietic cells to mediate cell-cell interactions. CD169 is expressed on a subset of macrophages and dendritic cells. On CD14+ monocytes its expression can be induced by interferon alpha and gamma. High expression of CD169 is observed in the spleen, lymph nodes, bone marrow, and under inflammatory conditions rheumatoid arthritis and atherosclerosis, lower in the liver, lungs and gut. It has been shown to be involved in antigen presentation to invariant NKT cells, which play an important role in the innate arm of the immune system to modulate the subsequent acquired immune responses. |
| Function | Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt] |
| Research Area | Immune System antibody; Microbiology and Infectious Disease antibody |
| Calculated MW | 183 kDa |
