ARG22870

anti-CD169 / Siglec 1 antibody [3D6.112]

anti-CD169 / Siglec 1 antibody [3D6.112] for CyTOF®-candidate,Flow cytometry,ICC/IF,IHC-Frozen sections and Mouse

Overview

Product Description Rat Monoclonal antibody [3D6.112] recognizes CD169 / Siglec 1
Tested Reactivity Ms
Tested Application CyTOF®-candidate, FACS, ICC/IF, IHC-Fr
Host Rat
Clonality Monoclonal
Clone 3D6.112
Isotype IgG2a
Target Name CD169 / Siglec 1
Antigen Species Mouse
Immunogen Purified murine sialoadhesin.
Conjugation Un-conjugated
Alternate Names CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1:100 - 1:1000
ICC/IFAssay-dependent
IHC-Fr1:50 - 1:100
Application Note IHC-Fr: Arigo recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20612 Mouse SIGLEC1

Swiss-port # Q62230 Mouse Sialoadhesin

Gene Symbol Siglec1
Gene Full Name sialic acid binding Ig-like lectin 1, sialoadhesin
Background This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]
Function Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt]
Highlight Related products:
CD169 antibodies; CD169 ELISA Kits; Anti-Rat IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Calculated MW 183 kDa

Images (6) Click the Picture to Zoom In

  • ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112] IHC-Fr image

    Immunohistochemistry: Frozen section of Mouse spleen stained with ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112].

  • ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112] followed by Goat anti Rat IgG antibody as a detection reagent. (Low power).

  • ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112] followed by Goat anti Rat IgG antibody as a detection reagent. (Medium power).

  • ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112] followed by Goat anti Rat IgG antibody as a detection reagent. (High power).

  • ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112], green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI. (Low power).

  • ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22870 anti-CD169 / Siglec 1 antibody [3D6.112], green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI. (High power).