ARG42425

anti-CD164 antibody [67D2]

anti-CD164 antibody [67D2] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [67D2] recognizes CD164
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IP, WB
Specificity The mouse monoclonal antibody 67D2 recognizes an extracellular class III epitope (not sensitive to sialidase, N-glycanase, O-glycosidase, and O-sialoglycoprotease) of CD164, a sialomucin expressed in hematopoietic myeloid and erythroid progenitors, activated basophils, and in various carcinomas and leukemic cells.
Host Mouse
Clonality Monoclonal
Clone 67D2
Isotype IgG1
Target Name CD164
Antigen Species Human
Immunogen Breast tumor cell line T-47D.
Conjugation Un-conjugated
Alternate Names MGC-24; Multi-glycosylated core protein 24; CD antigen CD164; Endolyn; endolyn; Sialomucin core protein 24; MGC-24v; MUC-24

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8763 Human CD164

Swiss-port # Q04900 Human Sialomucin core protein 24

Gene Symbol CD164
Gene Full Name CD164 molecule, sialomucin
Background This gene encodes a transmembrane sialomucin and cell adhesion molecule that regulates the proliferation, adhesion and migration of hematopoietic progenitor cells. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing. [provided by RefSeq, Oct 2016]
Function Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34(+) cells to the stroma and by negatively regulating CD34(+)CD38(lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediated through the CXCL12/CXCR4 axis. May play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells. Promotes myogenesis by enhancing CXCR4-dependent cell motility. Positively regulates myoblast migration and promotes myoblast fusion into myotubes (By similarity). [UniProt]
Cellular Localization Lysosome membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt]
Calculated MW 21 kDa
PTM Highly N- and O-glycosylated; contains sialic acid.

The motif Ser-Gly may serve as the site of attachment of a glycosaminoglycan side chain. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG42425 anti-CD164 antibody [67D2] FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG42425 anti-CD164 antibody [67D2], followed by FITC-conjugated Goat anti-Mouse antibody.