ARG45421
anti-CD162 / PSGL1 antibody
anti-CD162 / PSGL1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD162 / PSGL1 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD162 / PSGL1 |
| Antigen Species | Mouse |
| Immunogen | Recombinant protein containing to mouse CD162 / PSGL1. |
| Conjugation | Un-conjugated |
| Alternate Names | SELPLG; Selectin P ligand; PSGL1; P-selectin glycoprotein ligand 1; PSGL-1; CD162; CLA; CD antigen CD162 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 110 - 120 kDa |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | SELPLG |
|---|---|
| Gene Full Name | selectin P ligand |
| Background | CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors. |
| Function | A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture. [UniProt]. [UniProt] |
| Cellular Localization | Cell membrane. [UniProt] |
| Calculated MW | 43 kDa |
| PTM | Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation. Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45421 anti-CD162 / PSGL1 antibody FACS image
Flow Cytometry: NRK stained with ARG45421 anti-CD162 / PSGL1 antibody at 1 µg/10^6 cells dilution.
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ARG45421 anti-CD162 / PSGL1 antibody IHC-P image
Immunohistochemistry: Rat spleen stained with ARG45421 anti-CD162 / PSGL1 antibody at 1 μg/ml dilution.
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ARG45421 anti-CD162 / PSGL1 antibody WB image
Western blot: Rat thymus stained with ARG45421 anti-CD162 / PSGL1 antibody at 0.5 μg/ml dilution.
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ARG45421 anti-CD162 / PSGL1 antibody IHC-P image
Immunohistochemistry: Mouse intestine stained with ARG45421 anti-CD162 / PSGL1 antibody at 1 μg/ml dilution.
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ARG45421 anti-CD162 / PSGL1 antibody WB image
Western blot: Mouse thymus stained with ARG45421 anti-CD162 / PSGL1 antibody at 0.5 μg/ml dilution.
