ARG22504
anti-CD161 antibody [PK136] (azide free)
anti-CD161 antibody [PK136] (azide free) for Flow cytometry,Functional study,Immunoprecipitation and Mouse
Overview
| Product Description | Azide free Mouse Monoclonal antibody [PK136] recognizes CD161 This antibody recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. |
|---|---|
| Tested Reactivity | Ms |
| Species Does Not React With | Hu, Rat |
| Tested Application | FACS, FuncSt, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PK136 |
| Isotype | IgG2a |
| Target Name | CD161 |
| Antigen Species | Mouse |
| Immunogen | Spleen and bone marrow cells from CE mice. |
| Conjugation | Un-conjugated |
| Alternate Names | CLEC5B; CD antigen CD161; CD161; NKR-P1; NKR-P1A; Killer cell lectin-like receptor subfamily B member 1; NKRP1A; NKR; HNKR-P1a; Natural killer cell surface protein P1A; C-type lectin domain family 5 member B; hNKR-P1A |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q0ZUP1 Mouse Killer cell lectin-like receptor subfamily B member 1 |
|---|---|
| Gene Symbol | Klrb1b |
| Gene Full Name | killer cell lectin-like receptor subfamily B member 1B |
| Background | Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. [provided by RefSeq, Jul 2008] |
| Function | Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | N-glycosylated. Contains sialic acid residues. |
