ARG23291
anti-CD16 antibody [KD1]
anti-CD16 antibody [KD1] for Flow cytometry,Functional study and Human,Bovine,Sheep
Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
Overview
| Product Description | Mouse Monoclonal antibody [KD1] recognizes CD16 Mouse anti Human CD16 antibody, clone KD1 recognizes human CD16, a 50-65 kDa cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages (Moretta et al. 1990), whereas CD16B is primarily expressed by granulocytes (Bonecchi et al. 1999). Clone KD1 recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16. Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine (Boysen et al. 2010) and ovine (Elhmouzi-Younes et al. 2010). |
|---|---|
| Tested Reactivity | Hu, Bov, Sheep |
| Species Does Not React With | Rat, Dog |
| Tested Application | FACS, FuncSt |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | KD1 |
| Isotype | IgG2a |
| Target Name | CD16 |
| Antigen Species | Human |
| Immunogen | A polyclonal population of NK cells. |
| Conjugation | Un-conjugated |
| Alternate Names | FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII |
Application Instructions
| Application Suggestion |
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| Application Note | Functional assay: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. FACS: Use 100 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08637 Human Low affinity immunoglobulin gamma Fc region receptor III-A |
|---|---|
| Gene Symbol | FCGR3A |
| Gene Full Name | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
| Background | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt] |
| Highlight | Related products: CD16 antibodies; CD16 ELISA Kits; CD16 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody |
| Calculated MW | 29 kDa |
| PTM | Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms. The soluble form is produced by a proteolytic cleavage. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG23291 anti-CD16 antibody [KD1] FACS image
Flow Cytometry: Human peripheral blood lymphocytes stained with ARG23291 anti-CD16 antibody [KD1] followed by Rabbit F(ab')2 anti Mouse IgG (FITC).
