ARG45668
anti-CD155 / Poliovirus Receptor antibody [5I13D1]
anti-CD155 / Poliovirus Receptor antibody [5I13D1] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody [5I13D1] recognizes CD155 / Poliovirus Receptor |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5I13D1 |
| Isotype | IgG2a |
| Target Name | CD155 / Poliovirus Receptor |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human CD155 / Poliovirus Receptor . |
| Conjugation | Un-conjugated |
| Alternate Names | PVR; NECL5; CD antigen CD155; Nectin-like protein 5; PVS; Necl-5; HVED; Poliovirus receptor; TAGE4; NECL-5; CD155 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 70 - 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PVR |
| Gene Full Name | poliovirus receptor |
| Background | The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
| Function | Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. [UniProt] |
| Cellular Localization | Isoform Alpha: Cell membrane; Single-pass type I membrane protein. Isoform Delta: Cell membrane; Single-pass type I membrane protein. Isoform Beta: Secreted. Isoform Gamma: Secreted. [UniProt] |
| Calculated MW | 45 kDa |
| PTM | N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4. Phosphorylated by Src kinases on tyrosine residues in the ITIM motif upon ligation. Interaction with TIGIT is required for Phosphorylation. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45668 anti-CD155 / Poliovirus Receptor antibody [5I13D1] IHC-P image
Immunohistochemistry: Human placenta stained with ARG45668 anti-CD155 / Poliovirus Receptor antibody [5I13D1] at 2 μg/ml dilution.
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ARG45668 anti-CD155 / Poliovirus Receptor antibody [5I13D1] WB image
Western blot: A549 stained with ARG45668 anti-CD155 / Poliovirus Receptor antibody [5I13D1] at 0.5 μg/ml dilution.
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ARG45668 anti-CD155 / Poliovirus Receptor antibody [5I13D1] FACS image
Flow Cytometry: PC-3 stained with ARG45668 anti-CD155 / Poliovirus Receptor antibody [5I13D1] at 1 µg/10^6 cells dilution.
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ARG45668 anti-CD155 / Poliovirus Receptor antibody [5I13D1] IHC-P image
Immunohistochemistry: Mouse liver stained with ARG45668 anti-CD155 / Poliovirus Receptor antibody [5I13D1] at 2 μg/ml dilution.
