ARG41609

anti-CD154 / CD40L antibody [CD40LG/2761]

anti-CD154 / CD40L antibody [CD40LG/2761] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Mouse Monoclonal antibody [CD40LG/2761] recognizes CD154 / CD40L
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P
Host Mouse
Clonality Monoclonal
Clone CD40LG/2761
Isotype IgG2b, kappa
Target Name CD154 / CD40L
Antigen Species Human
Immunogen Protein fragment corresponding to aa. 108-261 of Human CD154.
Conjugation Un-conjugated
Alternate Names TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 959 Human CD40LG

Swiss-port # P29965 Human CD40 ligand

Gene Symbol CD40LG
Gene Full Name CD40 ligand
Background The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Function Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.

Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt]
Cellular Localization Cell membrane; Single-pass type II membrane protein. Cell surface. CD40 ligand, soluble form: Secreted. [UniProt]
Calculated MW 29 kDa
PTM The soluble form derives from the membrane form by proteolytic processing.

N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.

Not O-glycosylated. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] ICC/IF image

    Immunofluorescence: Paraformaldehyde-fixed Jurkat cells stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] (green) and Reddot nuclear stain (red).

  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human spleen tissue stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761]. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.

  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] FACS image

    Flow Cytometry: Jurkat cells stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] (blue) or isotype control antibody (red), followed by incubation with FITC labelled secondary antibody.

  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human spleen tissue stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761]. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.

  • ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained with ARG41609 anti-CD154 / CD40L antibody [CD40LG/2761]. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.