ARG66058

anti-CD154 / CD40L antibody (Biotin)

anti-CD154 / CD40L antibody (Biotin) for ELISA,Western blot and Human

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes CD154 / CD40L
Tested Reactivity Hu
Tested Application ELISA, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CD154 / CD40L
Antigen Species Human
Immunogen E. coli derived recombinant Human CD154 / CD40L.
(MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL)
Conjugation Biotin
Alternate Names TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66057 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 959 Human CD40LG

Swiss-port # P29965 Human CD40 ligand

Gene Symbol CD40LG
Gene Full Name CD40 ligand
Background The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Function Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.

Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt]
Calculated MW 29 kDa
PTM The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.

Images (4) Click the Picture to Zoom In

  • ARG66058 anti-CD154 / CD40L antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human sCD40L stained with ARG66058 anti-CD154 / CD40L antibody (Biotin), under non-reducing conditions.

  • ARG66058 anti-CD154 / CD40L antibody (Biotin) standard curve image

    Direct ELISA: ARG66058 anti-CD154 / CD40L antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66058 anti-CD154 / CD40L antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human sCD40L stained with ARG66058 anti-CD154 / CD40L antibody (Biotin), under reducing conditions.

  • ARG66058 anti-CD154 / CD40L antibody (Biotin) standard curve image

    Sandwich ELISA: ARG66058 anti-CD154 / CD40L antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66057 anti-CD154 / CD40L antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.