ARG66057

anti-CD154 / CD40L antibody

anti-CD154 / CD40L antibody for ELISA,Western blot and Human

Overview

Product Description Goat Polyclonal antibody recognizes CD154 / CD40L
Tested Reactivity Hu
Tested Application ELISA, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CD154 / CD40L
Antigen Species Human
Immunogen E. coli derived recombinant Human CD154 / CD40L.
(MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL)
Conjugation Un-conjugated
Alternate Names TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66058 as a detection antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 959 Human CD40LG

Swiss-port # P29965 Human CD40 ligand

Gene Symbol CD40LG
Gene Full Name CD40 ligand
Background The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Function Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.

Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt]
Calculated MW 29 kDa
PTM The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.

Images (3) Click the Picture to Zoom In

  • ARG66057 anti-CD154 / CD40L antibody WB image

    Western blot: 250 - 0.24 ng of Human sCD40-Ligand stained with ARG66057 anti-CD154 / CD40L antibody, under non-reducing conditions.

  • ARG66057 anti-CD154 / CD40L antibody standard curve image

    Sandwich ELISA: ARG66057 anti-CD154 / CD40L antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66058 anti-CD154 / CD40L antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66057 anti-CD154 / CD40L antibody WB image

    Western blot: 250 - 0.24 ng of Human sCD40-Ligand stained with ARG66057 anti-CD154 / CD40L antibody, under reducing conditions.