ARG65420

anti-CD152 / CTLA4 antibody [BNI3] (low endotoxin)

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100 μg

389.00

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Datasheet
Publication

Overview

Product Description	Azide free and low endotoxin Mouse Monoclonal antibody [BNI3] recognizes CD152 / CTLA4
Tested Reactivity	Hu
Tested Application	FACS, ICC/IF, IHC-Fr, IP
Specificity	The mouse monoclonal antibody BNI3 recognizes human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.
Host	Mouse
Clonality	Monoclonal
Clone	BNI3
Isotype	IgG2a
Target Name	CD152 / CTLA4
Antigen Species	Human
Immunogen	Human CD152-IgG heavy chain fusion protein
Conjugation	Un-conjugated
Alternate Names	GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	1 - 4 µg/ml
ICC/IF	Assay-dependent
IHC-Fr	Assay-dependent
IP	Assay-dependent

Application Note

FACS: Intracellular staining.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein A.
Purification Note	0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test.
Purity	> 95% (by SDS-PAGE)
Buffer	PBS (pH 7.4)
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 1493 Human CTLA4 Swiss-port # P16410 Human Cytotoxic T-lymphocyte protein 4
Gene Symbol	CTLA4
Gene Full Name	cytotoxic T-lymphocyte-associated protein 4
Background	CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.
Function	Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
Research Area	Developmental Biology antibody; Immune System antibody
Calculated MW	25 kDa
PTM	N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.

Images (3) Click the Picture to Zoom In

ARG65420 anti-CD152 / CTLA4 antibody [BNI3] (low endotoxin) FACS image

Flow Cytometry: PHA stimulated human peripheral whole blood stained with ARG65420 anti-CD152 / CTLA4 antibody [BNI3] (low endotoxin) at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG65420 anti-CD152 / CTLA4 antibody [BNI3] (low endotoxin) FACS image

Flow Cytometry: Human PHA stimulated lymphocytes stained with ARG65420 anti-CD152 / CTLA4 antibody [BNI3] (low endotoxin) at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody. Cells were co-stained with ARG53820 anti-CD3 antibody [UCHT1] (PE) (20 µl reagent / 100 µl of peripheral whole blood).
ARG65420 anti-CD152 / CTLA4 antibody [BNI3] (low endotoxin) FACS image

Flow Cytometry: Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65420 anti-CD152 / CTLA4 antibody [BNI3] (low endotoxin) at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.