ARG42299
anti-CD152 / CTLA4 antibody [BNI3] (APC)
anti-CD152 / CTLA4 antibody [BNI3] (APC) for Flow cytometry and Human
Overview
| Product Description | APC-conjugated Mouse Monoclonal antibody [BNI3] recognizes CD152 / CTLA4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody BNI3 recognizes an extracellular domain of human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BNI3 |
| Isotype | IgG2a |
| Target Name | CD152 / CTLA4 |
| Antigen Species | Human |
| Immunogen | Human CD152-IgG heavy chain fusion protein. |
| Conjugation | APC |
| Alternate Names | GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTLA4 |
| Gene Full Name | cytotoxic T-lymphocyte-associated protein 4 |
| Background | This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008] |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG42299 anti-CD152 / CTLA4 antibody [BNI3] (APC) FACS image
Flow Cytometry: PHA stimulated human peripheral whole blood stained with ARG42299 anti-CD152 / CTLA4 antibody [BNI3] (APC) (10 µl reagent / 100 µl of peripheral whole blood).
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ARG42299 anti-CD152 / CTLA4 antibody [BNI3] (APC) FACS image
Flow Cytometry: PHA stimulated human lymphocytes stained with ARG42299 anti-CD152 / CTLA4 antibody [BNI3] (APC) (10 µl reagent / 100 µl of peripheral whole blood) and ARG53820 anti-CD3 antibody [UCHT1] (PE) (20 µl reagent / 100 µl of peripheral whole blood).
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ARG42299 anti-CD152 / CTLA4 antibody [BNI3] (APC) FACS image
Flow Cytometry: Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG42299 anti-CD152 / CTLA4 antibody [BNI3] (APC) (10 µl reagent / 100 µl of peripheral whole blood).
