ARG23611

anti-CD150 / SLAM antibody [9D1] (low endotoxin)

anti-CD150 / SLAM antibody [9D1] (low endotoxin) for Flow cytometry,Functional study and Mouse

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [9D1] recognizes CD150 / SLAM.
This product recognizes murine CD150, also known as signalling lymphocyte activation molecule (SLAM) . Murine CD150 is a 75 kDa cell surface glycoprotein that is expressed on T-cells and B-cells, and is upregulated on activated T-cells and stimulated macrophages. The extracellular domain of CD150 is the receptor for the measles virus (Erlenhoefer et al. 2001) and acts as a co-activator on T-cells and B-cells.Rat anti Mouse CD150 antibody, clone 9D1 has functional activity (Howie et al. 2002).
Tested Reactivity Ms
Tested Application FACS, FuncSt
Host Rat
Clonality Monoclonal
Clone 9D1
Isotype IgG1
Target Name CD150 / SLAM
Antigen Species Mouse
Immunogen CHO cells stably transfected with Mouse CD150.
Conjugation Un-conjugated
Alternate Names Signaling lymphocytic activation molecule; IPO-3; CD150; SLAM; CDw150; CD antigen CD150

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25 - 1:100
FuncStAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note Low endotoxin
Buffer PBS.
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27218 Mouse SLAMF1

Swiss-port # Q9QUM4 Mouse Signaling lymphocytic activation molecule

Gene Symbol SLAMF1
Gene Full Name signaling lymphocytic activation molecule family member 1
Function High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. [UniProt]
Calculated MW 37 kDa
PTM Phosphorylated on tyrosine residues by FYN. [UniProt]