ARG62735
anti-CD15 antibody [MEM-158]
anti-CD15 antibody [MEM-158] for CyTOF®-candidate,Flow cytometry,Immunoprecipitation and Human
Controls and Markers antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-158] recognizes CD15 |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Bov, Pig, Sheep |
| Tested Application | CyTOF®-candidate, FACS, IP |
| Specificity | The clone MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. HLDA VI; WS Code AS A053 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-158 |
| Isotype | IgM |
| Target Name | CD15 |
| Antigen Species | Human |
| Immunogen | Human granulocytes |
| Conjugation | Un-conjugated |
| Alternate Names | LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by precipitation methods and ion exchange chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FUT4 |
| Gene Full Name | fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) |
| Background | CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease. |
| Function | May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt] |
| Research Area | Controls and Markers antibody |
| Calculated MW | 59 kDa |
Images (3) Click the Picture to Zoom In
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ARG62735 anti-CD15 antibody [MEM-158] FACS image
Flow Cytometry: Human peripheral blood stained with ARG62735 anti-CD15 antibody [MEM-158] at 0.3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62735 anti-CD15 antibody [MEM-158] FACS image
Flow Cytometry: Separation of human neutrophil granulocytes (red-filled) from CD15 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62735 anti-CD15 antibody [MEM-158] at 0.3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62735 anti-CD15 antibody [MEM-158] CyTOF image
CyTOF: Human peripheral blood cells (after ammonium chloride red blood cell lysis) stained with ARG62735 anti-CD15 antibody [MEM-158] (173Yb). Singlet cells were gated for data analysis.
