ARG42487

anti-CD15 antibody

anti-CD15 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CD15
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD15
Antigen Species Human
Immunogen Synthetic peptide within aa. 200-300 of Human CD15 (NP_002024.1).
Conjugation Un-conjugated
Alternate Names LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 59 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14345 Mouse FUT4

GeneID: 2526 Human FUT4

Swiss-port # P22083 Human Alpha-(1,3)-fucosyltransferase 4

Swiss-port # Q11127 Mouse Alpha-(1,3)-fucosyltransferase 4

Gene Symbol FUT4
Gene Full Name fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
Background The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
Function May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt]
Cellular Localization Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. [UniProt]
Calculated MW 59 kDa

Images (4) Click the Picture to Zoom In

  • ARG42487 anti-CD15 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon tissue stained with ARG42487 anti-CD15 antibody at 1:100 dilution.

  • ARG42487 anti-CD15 antibody WB image

    Western blot: 25 µg of HL-60 cell lysate stained with ARG42487 anti-CD15 antibody at 1:1000 dilution.

  • ARG42487 anti-CD15 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue stained with ARG42487 anti-CD15 antibody at 1:100 dilution.

  • ARG42487 anti-CD15 antibody IHC image

    Immunohistochemistry: Paraffin-embedded Human colon carcinoma tissue stained with ARG42487 anti-CD15 antibody at 1:100 dilution.