ARG65505
anti-CD148 / DEP1 antibody [MEM-CD148 / 05] (Biotin)
anti-CD148 / DEP1 antibody [MEM-CD148 / 05] (Biotin) for Flow cytometry and Human
Signaling Transduction antibody
Overview
| Product Description | Biotin-conjugated Mouse Monoclonal antibody [MEM-CD148/05] recognizes CD148 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The clone MEM-CD148/05 recognizes CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-CD148/05 |
| Isotype | IgG2b |
| Target Name | CD148 / DEP1 |
| Antigen Species | Human |
| Immunogen | Human recombinant CD148 (amino acids 1-444) |
| Conjugation | Biotin |
| Alternate Names | R-PTP-J; Density-enhanced phosphatase 1; R-PTP-ETA; DEP-1; SCC1; DEP1; CD antigen CD148; Receptor-type tyrosine-protein phosphatase eta; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; EC 3.1.3.48; HPTPeta; HPTP eta; R-PTP-eta; CD148 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q12913 Human Receptor-type tyrosine-protein phosphatase eta |
|---|---|
| Gene Symbol | PTPRJ |
| Gene Full Name | protein tyrosine phosphatase, receptor type, J |
| Background | CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes. |
| Function | Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 146 kDa |
| PTM | N- and O-glycosylated. |
Images (1) Click the Picture to Zoom In
-
ARG65505 anti-CD148 / DEP1 antibody [MEM-CD148 / 05] (Biotin) FACS image
Flow Cytometry: Separation of Human CD148 positive Granulocytes (red) from Human CD148 negative Lymphocytes (black-dashed). Human peripheral blood leukocytes stained with ARG65505 anti-CD148 / DEP1 antibody [MEM-CD148 / 05] (Biotin), followed by Streptavidin (APC).
