ARG23019
anti-CD14 antibody [CC-G33] (FITC)
anti-CD14 antibody [CC-G33] (FITC) for Flow cytometry and Human,Bovine,Sheep
Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
Overview
| Product Description | FITC-conjugated Mouse Monoclonal antibody [CC-G33] recognizes CD14 Mouse anti Bovine CD14, clone CC-G33 is a monoclonal antibody recognizing bovine CD14, a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells.CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.Mouse anti Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells, and also recognises an epitope on ovine CD14, see Sopp et al. 1996 for details. CC-G33 has also been shown to be reactive with CD14 from the Water buffalo (Bubalus bubalis), see Mirielli et al. 2013. |
|---|---|
| Tested Reactivity | Hu, Bov, Sheep |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CC-G33 |
| Isotype | IgG1 |
| Target Name | CD14 |
| Antigen Species | Bovine |
| Immunogen | Partially purified polypeptides isolated from Bovine leucocyte cell surface membrane. |
| Conjugation | FITC |
| Alternate Names | CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide and 1% BSA |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08571 Human Monocyte differentiation antigen CD14 Swiss-port # Q95122 Bovine Monocyte differentiation antigen CD14 |
|---|---|
| Gene Symbol | CD14 |
| Gene Full Name | CD14 molecule |
| Background | The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010] |
| Function | In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody |
| Calculated MW | 40 kDa |
| PTM | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
Images (1) Click the Picture to Zoom In
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ARG23019 anti-CD14 antibody [CC-G33] (FITC) FACS image
Flow Cytometry: Figure A. ARG23019 anti-CD14 antibody [CC-G33] (FITC) and RPE conjugated mouse IgG1 isotype control. Figure B. ARG23019 anti-CD14 antibody [CC-G33] (FITC) and RPE conjugated mouse anti bovine CD80. All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
