ARG54219
anti-CD138 / Syndecan 1 antibody [B-A38] (PE)
anti-CD138 / Syndecan 1 antibody [B-A38] (PE) for Flow cytometry and Human
Cancer antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [B-A38] recognizes CD138 / Syndecan 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The antibody BA38 recognizes CD138 (syndecan 1), a heparan sulfate proteoglycan protein expressed mainly in the epidermis and plasma cells, but also in growth factorstimulated lymphocytes. The MWs of the glycosylated CD138 were variety, and MW of the non-glycosylated core protein (dimmer form) was around 60-80 kda. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B-A38 |
| Isotype | IgG1 |
| Target Name | CD138 / Syndecan 1 |
| Antigen Species | Human |
| Immunogen | U266 human peripheral blood myeloma cell line |
| Conjugation | PE |
| Alternate Names | CD antigen CD138; syndecan; CD138; SDC; Syndecan-1; SYND1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SDC1 |
| Gene Full Name | syndecan 1 |
| Background | CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. |
| Function | Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. [UniProt] |
| Research Area | Cancer antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 32 kDa |
| PTM | Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. |
Clone References
KSHV attachment and entry are dependent on αVβ3 integrin localized to specific cell surface microdomains and do not correlate with the presence of heparan sulfate.
ICC/IF / Human
Heparan sulfate regulates hair follicle and sebaceous gland morphogenesis and homeostasis.
IHC-FoFr / Mouse
Flow cytometry assessment of in vitro generated CD138+ human plasma cells.
FACS / Human
Clinical implications in the shift of syndecan-1 expression from the cell membrane to the cytoplasm in bladder cancer.
IHC-P / Human
Glycocalyx protection reduces leukocyte adhesion after ischemia/reperfusion.
IHC-P / Guinea pig
Oxidative stress alters syndecan-1 distribution in lungs with pulmonary fibrosis.
IHC-P / Human, Mouse
