ARG66064
anti-CD137L / TNFSF9 antibody
anti-CD137L / TNFSF9 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD137L / TNFSF9 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD137L / TNFSF9 |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human TNFSF9. (MREGPELSPD DPAGLLDLRQ GMFAQLVAQN VLLIDGPLSW YSDPGLAGVS LTGGLSYKED TKELVVAKAG VYYVFFQLEL RRVVAGEGSG SVSLALHLQP LRSAAGAAAL ALTVDLPPAS SEARNSAFGF QGRLLHLSAG QRLGVHLHTE ARARHAWQLT QGATVLGLFR VTPEIPAGLP SPRSE) |
| Conjugation | Un-conjugated |
| Alternate Names | 4-1BBL; 4-1BB ligand; Tumor necrosis factor ligand superfamily member 9; 4-1BB-L; CD137L |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P41273 Human Tumor necrosis factor ligand superfamily member 9 |
|---|---|
| Gene Symbol | TNFSF9 |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 9 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.[provided by RefSeq, Oct 2008] |
| Function | Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. [UniProt] |
| Calculated MW | 27 kDa |
Images (4) Click the Picture to Zoom In
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ARG66064 anti-CD137L / TNFSF9 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon and rectum adenocarcinoma. The recommended ARG66064 anti-CD137L / TNFSF9 antibody concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.
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ARG66064 anti-CD137L / TNFSF9 antibody WB image
Western blot: 250 - 0.24 ng (left to right) of recombinant Human TNFSF9 stained with ARG66064 anti-CD137L / TNFSF9 antibody, under non-reducing conditions.
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ARG66064 anti-CD137L / TNFSF9 antibody standard curve image
Sandwich ELISA: ARG66064 anti-CD137L / TNFSF9 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66065 anti-TNFSF9 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66064 anti-CD137L / TNFSF9 antibody WB image
Western blot: 250 - 0.24 ng (left to right) of recombinant Human TNFSF9 stained with ARG66064 anti-CD137L / TNFSF9 antibody, under reducing conditions.
