ARG23097

anti-CD134 / OX40 antibody [OX-40]

anti-CD134 / OX40 antibody [OX-40] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Rat

Overview

Product Description Mouse Monoclonal antibody [OX-40] recognizes CD134 / OX40.
Mouse anti Rat CD134 antibody, clone OX-40 recognizes the OX40 antigen which is present on activated T lymphocytes that also express CD4 (Paterson et al. 1987).The antigen is a glycoprotein of approximately 50 kDa which is related in sequence to the low affinity Nerve Growth Factor Receptor (Mallett et al. 1990).Mouse anti Rat CD134 antibody, clone OX-40 is not mitogenic, but does enhance some T cell responses (Paterson et al. 1987).
Tested Reactivity Rat
Tested Application FACS, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone OX-40
Isotype IgG2b
Target Name CD134 / OX40
Antigen Species Rat
Immunogen Phytohemagglutinin (PHA) activated Rat Lymph Node Cells.
Conjugation Un-conjugated
Alternate Names CD134; TAX transcriptionally-activated glycoprotein 1 receptor; TXGP1L; OX40L receptor; Tumor necrosis factor receptor superfamily member 4; OX40; CD antigen CD134; IMD16; ACT35 antigen; ACT35

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25572 Rat TNFRSF4

Swiss-port # P15725 Rat Tumor necrosis factor receptor superfamily member 4

Gene Symbol Tnfrsf4
Gene Full Name tumor necrosis factor receptor superfamily, member 4
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation. [provided by RefSeq, Jul 2008]
Function Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity. [UniProt]
Calculated MW 29 kDa