ARG23136

anti-CD130 / gp130 antibody [B-T2]

anti-CD130 / gp130 antibody [B-T2] for ELISA,Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [B-T2] recognizes CD130 / gp130
Tested Reactivity Hu
Tested Application ELISA, FACS
Host Mouse
Clonality Monoclonal
Clone B-T2
Isotype IgG1
Target Name CD130 / gp130
Antigen Species Human
Immunogen Natural soluble gp130 receptor.
Conjugation Un-conjugated
Alternate Names CDw130; CD130; CDW130; Interleukin-6 signal transducer; CD antigen CD130; IL-6RB; Membrane glycoprotein 130; GP130; Oncostatin-M receptor subunit alpha; IL-6R subunit beta; Interleukin-6 receptor subunit beta; gp130; IL-6 receptor subunit beta; IL-6R-beta

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS0.2 µg/10^6 cells
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by ion exchange chromatography.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3572 Human IL6ST

Swiss-port # P40189 Human Interleukin-6 receptor subunit beta

Gene Symbol IL6ST
Gene Full Name interleukin 6 signal transducer
Background The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]
Function Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. [UniProt]
Calculated MW 104 kDa
PTM Phosphorylation of Ser-782 down-regulates cell surface expression.

Heavily N-glycosylated (PubMed:11098061, PubMed:16335952, PubMed:19159218, PubMed:19139490, PubMed:11251120). Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding (PubMed:19915009). [UniProt]