ARG65493

anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free)

anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) for Flow cytometry,Functional study,Immunoprecipitation and Human

Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Azide free Mouse Monoclonal antibody [MEM-25] recognizes CD11a / LFA1 alpha
Tested Reactivity Hu
Tested Application FACS, FuncSt, IP
Specificity The clone MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
HLDA IV; WS Code NL 209
Host Mouse
Clonality Monoclonal
Clone MEM-25
Isotype IgG1
Target Name CD11a / LFA1 alpha
Immunogen Leukocytes from a pacient suffering from a LGL-type leukaemia.
Conjugation Un-conjugated
Alternate Names Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 µg/ml
FuncStAssay-dependent
IPAssay-dependent
Application Note IP: Excellent antibody for immunoaffinity purification of LFA-1 complex.
Functional studies: The clone MEM-25 partially blocks binding of LFA-1 complex to ICAM-1.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3683 Human ITGAL

Swiss-port # P20701 Human Integrin alpha-L

Gene Symbol ITGAL
Gene Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Background CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Function Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 129 kDa
PTM In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents.

Images (2) Click the Picture to Zoom In

  • ARG65493 anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG65493 anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG65493 anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) FACS image

    Flow Cytometry: Separation of human monocytes (red-filled) from blood debris (black-dashed). Human peripheral whole blood stained with ARG65493 anti-CD11a / LFA1 alpha antibody [MEM-25] (azide free) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

Clone References

Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein.

Bazil V et al.
Folia Biol (Praha).,  (1990)

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