ARG54204
anti-CD116 antibody [4H1] (PE)
anti-CD116 antibody [4H1] (PE) for Flow cytometry and Human
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [4H1] recognizes CD116 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The clone 4H1 recognizes human CD116, the GM-CSF receptor alpha subunit (approx. 80 kDa) expressed e.g. by neutrophils, eosinophils, monocytes and macrophages. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4H1 |
| Isotype | IgG1 |
| Target Name | CD116 |
| Immunogen | CD116-transfected COS cells |
| Conjugation | PE |
| Alternate Names | Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; GMCSFR; CDw116; GM-CSF-R-alpha; CD antigen CD116; CSF2R; CD116; GMR-alpha; CSF2RAX; CSF2RAY; GMCSFR-alpha; GMR; SMDP4; CSF2RX; CSF2RY |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15509 Human Granulocyte-macrophage colony-stimulating factor receptor subunit alpha |
|---|---|
| Gene Symbol | CSF2RA |
| Gene Full Name | colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) |
| Background | CD116 (GM-CSF R alpha) is the low affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CD116 heterodimerizes with CD131, the common beta chain subunit shared with IL-3 and IL5 receptors, to form the high affinity GM-CSF receptor. CD116 is expressed by myeloid cells including macrophages, neutrophils, eosinophils, dendritic cells, and their precursors, as well as on endothelial cells. It is being used as a specific marker of myeloid leukemias. |
| Function | Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 46 kDa |
Images (2) Click the Picture to Zoom In
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ARG54204 anti-CD116 antibody [4H1] (PE) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG54204 anti-CD116 antibody [4H1] (PE) (10 µl reagent / 100 µl of peripheral whole blood).
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ARG54204 anti-CD116 antibody [4H1] (PE) FACS image
Flow Cytometry: Separation of human monocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG54204 anti-CD116 antibody [4H1] (PE) (10 µl reagent / 100 µl of peripheral whole blood).
